I-Human Papillomavirus (Izinhlobo ezingama-28) I-Genotyping Detection Kit (Fluorescence PCR)
Igama Lomkhiqizo
I-HWTS-CC004A-Human Papillomavirus (Izinhlobo ezingama-28) I-Genotyping Detection Kit (i-Fluorescence PCR)
I-Epidemiology
Ikhithi isebenzisa indlela yokuthola i-fluorescence ye-nucleic acid amplification (PCR) eminingi.Ama-primer akhethekile kakhulu kanye nama-probe aklanywe ngokususelwe kuchungechunge lwe-HPV yofuzo lwe-L1.Uphenyo oluthile lufakwe ilebula nge-FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore, HP435 , 45, 54, 56, 68, 82) kanye ne-ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) ngo-5', kanye neqembu le-3' quencher yi-BHQ1 noma i-BHQ2.Ngesikhathi sokukhulisa i-PCR, ama-primers athile nama-probe abophezela ngokulandelana kwawo okuqondiwe.Lapho i-enzyme ye-Taq ihlangana nama-probe aboshwe ngokulandelana okuqondiwe, isebenzisa umsebenzi we-5' end exonuclease ukuhlukanisa i-fluorophore yentatheli ku-quencher fluorophore, ukuze uhlelo lokuqapha i-fluorescence lukwazi ukuthola isignali ye-fluorescent, okungukuthi, njalo lapho i-DNA. i-strand iyakhuliswa, kwakheka i-molecule ye-fluorescent, eqaphela ukuvumelanisa okuphelele kokuqoqwa kwamasignali e-fluorescent kanye nokwakhiwa kwemikhiqizo ye-PCR, ukuze kuzuzwe ukutholwa kwekhwalithi kanye ne-genotyping yama-nucleic acid ezinhlobo ezingu-28 ze-papillomavirus yomuntu kumasampula eseli e-exfoliated yomlomo wesibeletho. .
Isiteshi
FAM | 16,58,53,73,6,26,40 |
VIC/HEX | 18,33,51,59,11,81,43 |
I-ROX | 31,66,52,39,83,61,42 |
CY5 | 56,35,45,68,54,44,82 |
Imingcele Yezobuchwepheshe
Isitoreji | ≤-18℃ ebumnyameni |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | Amaseli e-exfoliated womlomo wesibeletho |
Ct | ≤25 |
CV | ≤5.0% |
LoD | 25 amakhophi/ukusabela |
Izinsimbi Ezisebenzayo | I-Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)
I-Applied Biosystems 7500 Real-Time PCR Systems
Applied Biosystems 7500 Fast Real-Time PCR Systems
I-QuantStudio®5 Real-Time PCR Systems
SLAN-96P Real-Time PCR Systems
Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time
LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola
I-MA-6000 Real-Time Quantitative Thermal Cycler
I-BioRad CFX96 Real-Time PCR System
I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).