I-Human Papillomavirus (Izinhlobo ezingama-28) I-Genotyping Detection Kit (Fluorescence PCR)

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi kanye ne-genotyping ye-nucleic acid yezinhlobo ezingama-28 ze-human papillomavirus (HPV6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 43, 44, 45, 51, 52 , 53, 54, 56, 58, 59, 61, 66, 68, 73, 81, 82, 83) kumaseli e-exfoliated yomlomo wesibeletho wesifazane


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama Lomkhiqizo

I-HWTS-CC004A-Human Papillomavirus (Izinhlobo ezingama-28) I-Genotyping Detection Kit (i-Fluorescence PCR)

I-Epidemiology

Ikhithi isebenzisa indlela yokuthola i-fluorescence ye-nucleic acid amplification (PCR) eminingi.Ama-primer akhethekile kakhulu kanye nama-probe aklanywe ngokususelwe kuchungechunge lwe-HPV yofuzo lwe-L1.Uphenyo oluthile lufakwe ilebula nge-FAM fluorophore (HPV6, 16, 26, 40, 53, 58, 73), VIC/HEX fluorophore (HPV11, 18, 33, 43, 51, 59, 81), CY5 fluorophore, HP435 , 45, 54, 56, 68, 82) kanye ne-ROX fluorophore (HPV31, 39, 42, 52, 61, 66, 83) ngo-5', kanye neqembu le-3' quencher yi-BHQ1 noma i-BHQ2.Ngesikhathi sokukhulisa i-PCR, ama-primers athile nama-probe abophezela ngokulandelana kwawo okuqondiwe.Lapho i-enzyme ye-Taq ihlangana nama-probe aboshwe ngokulandelana okuqondiwe, isebenzisa umsebenzi we-5' end exonuclease ukuhlukanisa i-fluorophore yentatheli ku-quencher fluorophore, ukuze uhlelo lokuqapha i-fluorescence lukwazi ukuthola isignali ye-fluorescent, okungukuthi, njalo lapho i-DNA. i-strand iyakhuliswa, kwakheka i-molecule ye-fluorescent, eqaphela ukuvumelanisa okuphelele kokuqoqwa kwamasignali e-fluorescent kanye nokwakhiwa kwemikhiqizo ye-PCR, ukuze kuzuzwe ukutholwa kwekhwalithi kanye ne-genotyping yama-nucleic acid ezinhlobo ezingu-28 ze-papillomavirus yomuntu kumasampula eseli e-exfoliated yomlomo wesibeletho. .

Isiteshi

FAM

16,58,53,73,6,26,40

VIC/HEX

18,33,51,59,11,81,43

I-ROX 31,66,52,39,83,61,42
CY5 56,35,45,68,54,44,82

Imingcele Yezobuchwepheshe

Isitoreji ≤-18℃ ebumnyameni
Impilo yeshelufu

Izinyanga ezingu-12

Uhlobo lwesifanekiso Amaseli e-exfoliated womlomo wesibeletho
Ct ≤25
CV

≤5.0%

LoD

25 amakhophi/ukusabela

Izinsimbi Ezisebenzayo I-Easy Amp Real-time Fluorescence Isothermal Detection System(HWTS1600)

 

I-Applied Biosystems 7500 Real-Time PCR Systems

 

Applied Biosystems 7500 Fast Real-Time PCR Systems

 

I-QuantStudio®5 Real-Time PCR Systems

 

SLAN-96P Real-Time PCR Systems

 

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

 

LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

 

I-MA-6000 Real-Time Quantitative Thermal Cycler

 

I-BioRad CFX96 Real-Time PCR System

 

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Inketho 1.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).

Inketho yesi-2.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).


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