Ikhithi ye-RT-PCR yokuthola izinhlobo eziyisithupha zamagciwane okuphefumula (i-Fluorescence PCR)

Incazelo emfushane:

Le khithi ingasetshenziswa ukuthola ngokufanelekile i-nucleic acid ye-SARS-CoV-2, igciwane lomkhuhlane A, igciwane lomkhuhlane B, i-adenovirus, i-mycoplasma pneumoniae kanye negciwane lokuphefumula le-sycytial in vitro.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-OT058A/B/C/Z-Real time fluorescent RT-PCR kit yokuthola izinhlobo eziyisithupha zamagciwane okuphefumula

Isitifiketi

CE

I-Epidemiology

I-Corona Virus Disease 2019, ebizwa ngokuthi “i-COVID-19”, isho inyumoniya ebangelwa ukutheleleka kwe-SARS-CoV-2.I-SARS-CoV-2 iyi-coronavirus yohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi inani labantu ngokuvamile liyathinteka.Njengamanje, umthombo wokutheleleka ikakhulukazi iziguli ezingenwe yi-SARS-CoV-2, futhi abantu abangenwe yi-asymptomatic bangase babe umthombo wokutheleleka.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7.Umkhuhlane, ukukhwehlela okomile nokukhathala yizimpawu eziyinhloko.Iziguli ezimbalwa zazinokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo.

Umkhuhlane, owaziwa ngokuthi "umkhuhlane", isifo esithathelwanayo sokuphefumula esibangelwa igciwane lomkhuhlane.Iyathelelana kakhulu.Isakazwa kakhulu ngokukhwehlela kanye nokuthimula.Ivamise ukuqubuka entwasahlobo nasebusika.Amagciwane omkhuhlane we-influenza ahlukaniswe abe umkhuhlane A, IFV A, umkhuhlane B, IFV B, kanye no-C, IFV C izinhlobo ezintathu, zonke zingezamagciwane anamathelayo, abangela isifo somuntu ikakhulukazi kumagciwane omkhuhlane A no-B, yi-single-stranded, i-RNA virus ehlukene.Igciwane loMkhuhlane A liwukutheleleka kokuphefumula okunamandla, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, ezivame ukuguquka nokuqubuka emhlabeni jikelele.I-"Shift" ibhekisela ekuguqulweni kwegciwane lomkhuhlane A, okuholela ekuveleni kwegciwane elisha "i-subtype".Amagciwane oMkhuhlane B ahlukaniswe izigaba ezimbili, i-Yamagata ne-Victoria.Igciwane lomkhuhlane B line-antigenic drift kuphela, futhi ligwema ukubhekwa nokuqedwa kwamasosha omzimba ngokuguqulwa kwawo.Kodwa-ke, ijubane lokuvela kwegciwane lomkhuhlane B lihamba kancane kunelo legciwane le-human influenza A.Igciwane loMkhuhlane B lingase futhi libangele izifo zokuphefumula komuntu futhi libangele izifo eziwumshayabhuqe.

I-Adenovirus (AdV) ingeye-mammalian adenovirus, okuyigciwane le-DNA elinemiphetho ephindwe kabili ngaphandle kwemvilophu.Okungenani ama-genotypes angu-90 atholiwe, angahlukaniswa abe i-AG 7 subgenera.Ukutheleleka kwe-AdV kungabangela izifo ezihlukahlukene, okuhlanganisa inyumoniya, i-bronchitis, i-cystitis, i-eye conjunctivitis, izifo zesisu kanye ne-encephalitis.Inyumoniya ye-Adenovirus ingenye yezinhlobo ezinzima kakhulu zenyumoniya ezitholwa umphakathi ezinganeni, ezibalelwa ku-4% -10% wenyumoniya etholwa umphakathi.

I-Mycoplasma pneumoniae (MP) iwuhlobo lwe-microorganism encane kakhulu ye-prokaryotic, ephakathi kwebhaktheriya negciwane, enokwakheka kwamaseli kodwa alukho udonga lweseli.I-MP ikakhulukazi ibangela ukutheleleka komgudu wokuphefumula womuntu, ikakhulukazi ezinganeni nasebancane.Kungabangela inyumoniya ye-mycoplasma yomuntu, ukutheleleka kwezingane zokuphefumula kanye ne-atypical pneumonia.Izimpawu zomtholampilo zihlukahlukene, iningi lazo ukukhwehlela okukhulu, umkhuhlane, ukugodola, ikhanda elibuhlungu, umphimbo obuhlungu.Ukutheleleka komgudu wokuphefumula ophezulu kanye nenyumoniya ye-bronchial yizona ezivame kakhulu.Ezinye iziguli zingase zikhule zisuka ekuthelelekeni ngomgudu wokuphefumula ophezulu ziye kwinyumoniya enzima, ukucindezeleka okukhulu kokuphefumula nokufa kungase kwenzeke.

I-Respiratory syncytial virus (RSV) igciwane le-RNA, eliyingxenye yomndeni we-paramyxoviridae.Idluliselwa ngamaconsi omoya kanye nokuthintana eduze futhi iyi-pathogen eyinhloko yezifo eziphansi zokuphefumula ezinsaneni.Izinsana ezingenwe i-RSV zingase zibe ne-bronchiolitis enamandla (okubizwa ngokuthi i-bronchiolitis) kanye ne-pneumonia, ehlobene nesifo somoya ezinganeni.Izinsana zinezimpawu ezinzima, ezihlanganisa ukushisa okukhulu, i-rhinitis, i-pharyngitis ne-laryngitis, bese kuba yi-bronchiolitis ne-pneumonia.Izingane ezimbalwa ezigulayo zingaba yinkimbinkimbi nge-otitis media, i-pleurisy ne-myocarditis, njll.

Isiteshi

Igama lesiteshi I-R6 Rection Buffer A I-R6 Rection Buffer B
FAM I-SARS-CoV-2 I-HAdV
VIC/HEX Ukulawula Kwangaphakathi Ukulawula Kwangaphakathi
CY5 IFV A MP
I-ROX IFV B I-RSV

Imingcele Yezobuchwepheshe

Isitoreji ≤-18℃ ebumnyameni
Impilo yeshelufu 9 izinyanga
Uhlobo lwesifanekiso Igazi eliphelele, i-Plasma, iSerum
Ct ≤38
CV ≤5.0%
LoD 300Amakhophi/mL
Ukucaciswa Imiphumela ye-cross-reactivity ibonise ukuthi kwakungekho ukusabela okuphambene phakathi kwekhithi kanye ne-coronavirus yomuntu i-SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, i-rhinovirus A, B, C, i-chlamydia pneumoniae, i-metapneumovirus yomuntu, i-enterovirus A, B, C, D, igciwane le-human pulmonary, i-epstein-barr virus, igciwane lesimungumungwane, igciwane le-human cytomegalo, i-rotavirus, i-norovirus, i-parotitis virus, i-varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, s.pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci kanye ne-cryptococcus esanda kuzalwa kanye ne-nucleic acid yomuntu.
Izinsimbi Ezisebenzayo Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emakethe

SLAN-96P Real-Time PCR Systems
I-ABI 7500 Real-Time PCR Systems
ABI 7500 Fast Real-Time PCR Systems
I-QuantStudio®5 Real-Time PCR Systems
I-LightCycler®480 Real-Time PCR Systems
LineGene 9600 Plus Real-Time PCR Detection Systems
I-MA-6000 Real-Time Quantitative Thermal Cycler
I-BioRad CFX96 Real-Time PCR System, BioRad
Isistimu ye-PCR ye-CFX Opus 96 Yesikhathi Sangempela


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