I-Enterococcus emelana ne-Vancomycin kanye neGene Engazweli Emishanguzweni

Incazelo emfushane:

Le khithi isetshenziselwa ukutholwa kwekhwalithi ye-vancomycin-resistant enterococcus (VRE) kanye nofuzo olumelana nomuthi i-VanA ne-VanB esikhwehlela somuntu, igazi, umchamo noma amakoloni amsulwa.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-OT090-Vancomycin-resistant Enterococcus kanye ne-Gene Detection Kit (i-Fluorescence PCR)

I-Epidemiology

I-Resistance to Drug yaziwa nangokuthi ukumelana nomuthi, kubhekisela ekumelana namagciwane esenzweni semithi elwa namagciwane.Uma kwenzeka ukumelana nezidakamizwa, umphumela we-chemotherapy wezidakamizwa uzoncipha kakhulu.I-Resistance to Drug ihlukaniswe yaba ukumelana kwangaphakathi kanye nokumelana okutholakalayo.Ukumelana kwangaphakathi kunqunywa izakhi zofuzo ze-bacterial chromosomal, ezidluliselwa ezizukulwaneni ngezizukulwane, futhi ngeke ziguquke.Ukumelana okutholakalayo kungenxa yokuthi ngemva kokuthintana nama-antibiotics, amagciwane ashintsha izindlela zawo ze-metabolic ukuze angabulawa ama-antibiotics.

Izakhi zofuzo ezimelana ne-vancomycin i-VanA ne-VanB zitholwa ukumelana nomuthi, phakathi kwazo i-VanA ikhombisa amazinga aphezulu okumelana ne-vancomycin ne-teicoplanin, i-VanB ikhombisa amazinga ahlukene okumelana ne-vancomycin, futhi iyazwela ku-teicoplanin.I-Vancomycin ivamise ukusetshenziswa emtholampilo ukwelapha izifo ezibangelwa amagciwane e-Gram-positive, kodwa ngenxa yokuvela kwe-enterococci engazweli i-vancomycin (VRE), ikakhulukazi i-enterococcus faecalis kanye ne-enterococcus faecium, okubalelwa ngaphezu kuka-90%, ilethe izinselele ezintsha ekwelashweni komtholampilo. .Njengamanje, awukho umuthi othize we-antibacterial wokwelapha i-VRE.Ngaphezu kwalokho, i-VRE ingaphinde idlulisele izakhi zofuzo zokumelana nezidakamizwa kwamanye ama-enterococci noma amanye amagciwane e-Gram-positive.

Isiteshi

FAM I-Vancomycin-resistant enterococci (VRE): I-Enterococcus faecalis ne-Enterococcus faecium
VIC/HEX Ukulawula Kwangaphakathi
CY5 vancomycin ukumelana gene VanB
I-ROX vancomycin ukumelana gene VanA

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃
Impilo yeshelufu Izinyanga ezingu-12
Uhlobo lwesifanekiso isikhwehlela, igazi, umchamo noma amakoloni ahlanzekile
CV ≤5.0%
Ct ≤36
LoD 103I-CFU/mL
Ukucaciswa Ayikho i-cross-reactivity namanye amagciwane okuphefumula afana ne-klebsiella pneumoniae, i-acinetobacter baumannii, i-pseudomonas aeruginosa, i-streptococcus pneumoniae, i-neisseria meningitidis, i-staphylococcus aureus, i-klebsiella oxytoca, i-haemophilus aeruginosa, i-jumophilia, i-jumophilia, i-jumophilia, i-jumophilia, i-jumophilia. i-escherichia coli, i-pseudomonas fluorescens, i-candida albicans, i-chlamydia pneumoniae, i-adenovirus yokuphefumula, noma amasampula aqukethe ezinye izakhi zofuzo ezimelana nezidakamizwa i-CTX, i-mecA, i-SME, amasampula e-SHV ne-TEM.
Izinsimbi Ezisebenzayo I-Applied Biosystems 7500 Real-Time PCR System

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

Ukugeleza komsebenzi

Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .


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