I-Enterococcus emelana ne-Vancomycin kanye neGene Engazweli Emishanguzweni
Igama lomkhiqizo
I-HWTS-OT090-Vancomycin-resistant Enterococcus kanye ne-Gene Detection Kit (i-Fluorescence PCR)
I-Epidemiology
I-Resistance to Drug yaziwa nangokuthi ukumelana nomuthi, kubhekisela ekumelana namagciwane esenzweni semithi elwa namagciwane.Uma kwenzeka ukumelana nezidakamizwa, umphumela we-chemotherapy wezidakamizwa uzoncipha kakhulu.I-Resistance to Drug ihlukaniswe yaba ukumelana kwangaphakathi kanye nokumelana okutholakalayo.Ukumelana kwangaphakathi kunqunywa izakhi zofuzo ze-bacterial chromosomal, ezidluliselwa ezizukulwaneni ngezizukulwane, futhi ngeke ziguquke.Ukumelana okutholakalayo kungenxa yokuthi ngemva kokuthintana nama-antibiotics, amagciwane ashintsha izindlela zawo ze-metabolic ukuze angabulawa ama-antibiotics.
Izakhi zofuzo ezimelana ne-vancomycin i-VanA ne-VanB zitholwa ukumelana nomuthi, phakathi kwazo i-VanA ikhombisa amazinga aphezulu okumelana ne-vancomycin ne-teicoplanin, i-VanB ikhombisa amazinga ahlukene okumelana ne-vancomycin, futhi iyazwela ku-teicoplanin.I-Vancomycin ivamise ukusetshenziswa emtholampilo ukwelapha izifo ezibangelwa amagciwane e-Gram-positive, kodwa ngenxa yokuvela kwe-enterococci engazweli i-vancomycin (VRE), ikakhulukazi i-enterococcus faecalis kanye ne-enterococcus faecium, okubalelwa ngaphezu kuka-90%, ilethe izinselele ezintsha ekwelashweni komtholampilo. .Njengamanje, awukho umuthi othize we-antibacterial wokwelapha i-VRE.Ngaphezu kwalokho, i-VRE ingaphinde idlulisele izakhi zofuzo zokumelana nezidakamizwa kwamanye ama-enterococci noma amanye amagciwane e-Gram-positive.
Isiteshi
| FAM | I-Vancomycin-resistant enterococci (VRE): I-Enterococcus faecalis ne-Enterococcus faecium |
| VIC/HEX | Ukulawula Kwangaphakathi |
| CY5 | vancomycin ukumelana gene VanB |
| I-ROX | vancomycin ukumelana gene VanA |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | isikhwehlela, igazi, umchamo noma amakoloni ahlanzekile |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103I-CFU/mL |
| Ukucaciswa | Ayikho i-cross-reactivity namanye amagciwane okuphefumula afana ne-klebsiella pneumoniae, i-acinetobacter baumannii, i-pseudomonas aeruginosa, i-streptococcus pneumoniae, i-neisseria meningitidis, i-staphylococcus aureus, i-klebsiella oxytoca, i-haemophilus aeruginosa, i-jumophilia, i-jumophilia, i-jumophilia, i-jumophilia, i-jumophilia. i-escherichia coli, i-pseudomonas fluorescens, i-candida albicans, i-chlamydia pneumoniae, i-adenovirus yokuphefumula, noma amasampula aqukethe ezinye izakhi zofuzo ezimelana nezidakamizwa i-CTX, i-mecA, i-SME, amasampula e-SHV ne-TEM. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR System SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time |
Ukugeleza komsebenzi
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006C, HWTS-300) .
