I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid

Incazelo emfushane:

Ikhithi isetshenziselwa ukutholwa kwekhwalithi ye-MERS coronavirus nucleic acid ku-nasopharyngeal swabs ne-Middle East Respiratory Syndrome (MERS) coronavirus.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

I-Epidemiology

I-Middle East Respiratory Syndrome coronavirus (MERS-CoV), i-β-coronavirus ebangelaisifo sokuphefumula kubantu, saqala ukukhonjwa esigulini esineminyaka engu-60 sase-Saudi Arabia esishonile ngomhla ka-24th July, 2012. Isethulo somtholampilo sokutheleleka kwe-MERS-CoV sisukela esimweni esingabonakali noma izimpawu zokuphefumula ezincane kuya ezifweni ezinzima zokuphefumula ngisho nokufa.

Isiteshi

FAM Igciwane le-MERS RNA
I-VIC(HEX)

Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃ ebumnyameni

Impilo yeshelufu

9 izinyanga

Uhlobo lwesifanekiso

Ama-swabs e-nasopharyngeal asanda kuqoqwa

CV

≤5.0%

Ct

≤38

LoD

1000 Amakhophi/mL

Ukucaciswa

Akukho ukuhlangana kabusha nama-coronavirus abantu abantu i-SARSr-CoV namanye amagciwane ajwayelekile.

Izinsimbi Ezisebenzayo:

I-Applied Biosystems 7500 Real-Time PCR Systems

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Inketho 1.

Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.

Inketho yesi-2.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).


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