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    • I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid

    I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Isithombe Esifakiwe
    • I-Middle East Respiratory Syndrome Coronavirus Nucleic Acid

    Incazelo emfushane:

    Ikhithi isetshenziselwa ukutholwa kwekhwalithi ye-MERS coronavirus nucleic acid ku-nasopharyngeal swabs ne-Middle East Respiratory Syndrome (MERS) coronavirus.


    Imininingwane Yomkhiqizo

    Omaka bomkhiqizo

    Igama lomkhiqizo

    I-HWTS-RT031A-Middle East Respiratory Syndrome Coronavirus Nucleic Acid Detection Kit(Fluorescent PCR)

    I-Epidemiology

    I-Middle East Respiratory Syndrome coronavirus (MERS-CoV), i-β-coronavirus ebangelaisifo sokuphefumula kubantu, saqala ukukhonjwa esigulini esineminyaka engu-60 sase-Saudi Arabia esishonile ngomhla ka-24th July, 2012. Isethulo somtholampilo sokutheleleka kwe-MERS-CoV sisukela esimweni esingabonakali noma izimpawu zokuphefumula ezincane kuya ezifweni ezinzima zokuphefumula ngisho nokufa.

    Isiteshi

    FAM Igciwane le-MERS RNA
    I-VIC(HEX)

    Ukulawula Kwangaphakathi

    Imingcele Yezobuchwepheshe

    Isitoreji

    ≤-18℃ ebumnyameni

    Impilo yeshelufu

    9 izinyanga

    Uhlobo lwesifanekiso

    Ama-swabs e-nasopharyngeal asanda kuqoqwa

    CV

    ≤5.0%

    Ct

    ≤38

    LoD

    		 1000 Amakhophi/mL

    Ukucaciswa

    Akukho ukuhlangana kabusha nama-coronavirus abantu abantu i-SARSr-CoV namanye amagciwane ajwayelekile.

    Izinsimbi Ezisebenzayo:

    		 I-Applied Biosystems 7500 Real-Time PCR Systems

    Applied Biosystems 7500 Fast Real-Time PCR Systems

    I-QuantStudio®5 Real-Time PCR Systems

    SLAN-96P Real-Time PCR Systems

    Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

    LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

    I-MA-6000 Real-Time Quantitative Thermal Cycler

    I-BioRad CFX96 Real-Time PCR System

    I-BioRad CFX Opus 96 Real-Time PCR System

    Ukugeleza komsebenzi

    Inketho 1.

    Isisetshenziswa esisebenzayo sokumba esinconyiwe: I-QIAamp Viral RNA Mini Kit (52904), i-Nucleic Acid Extraction noma i-Purification Reagent (YDP315-R) yi-Tiangen Biotech (Beijing) Co., Ltd.

    Inketho yesi-2.

    Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).

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