Ukuguqulwa kwe-KRAS 8

Incazelo emfushane:

Le khithi ihloselwe ukutholwa kwekhwalithi ye-in vitro yezinguquko ezingu-8 kumakhodoni 12 no-13 ofuzo lwe-K-ras ku-DNA ekhishiwe ezigabeni zomuntu ezishumekwe ngupharafini.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-TM014-KRAS 8 Ikhithi Yokubona Ukuguquka (Fluorescence PCR)

I-HWTS-TM011-Ikhithi Yokubona Ukuguquka Kwenguquko ye-HWTS-TM011-KRAS 8(Fluorescence PCR)

Isitifiketi

CE

I-Epidemiology

Ukuguqulwa kwamaphuzu ofuzo lwe-KRAS kutholwe ezinhlotsheni eziningi zesimila somuntu, cishe u-17% ~ 25% izinga lokuguquka kwesimila, 15% ~ 30% izinga lokuguquka ezigulini ezinomdlavuza wamaphaphu, 20% ~ 50% izinga lokuguquka kumdlavuza we-colorectal. iziguli.Ngenxa yokuthi iphrotheni ye-P21 efakwe isakhi sofuzo se-K-ras itholakala ezansi nomfula we-EGFR womzila wokusayinda, ngemva kokuguqulwa kofuzo lwe-K-ras, indlela yokubonisa engezansi ihlale isebenza futhi ayithinteki izidakamizwa eziqondiswe phezulu ku-EGFR, okuholela ekuqhubekeleni phambili. ukwanda okuyingozi kwamaseli.Ukuguqulwa kwezakhi zofuzo ze-K-ras ngokuvamile kunikeza ukumelana ne-EGFR tyrosine kinase inhibitors ezigulini ezinomdlavuza wamaphaphu kanye nokumelana nezidakamizwa ezilwa ne-EGFR ezigulini ezinomdlavuza we-colorectal.Ngo-2008, iNational Comprehensive Cancer Network (NCCN) yakhipha umhlahlandlela wokusebenza komtholampilo womdlavuza we-colorectal, owaveza ukuthi amasayithi okushintshashintsha adala ukuthi ama-K-ras asebenze atholakala ikakhulukazi kuma-codon 12 no-13 e-exon 2, futhi yancoma ukuthi zonke iziguli ezinomdlavuza osezingeni eliphezulu we-metastatic colorectal zingahlolelwa ukuguqulwa kwe-K-ras ngaphambi kokwelashwa.Ngakho-ke, ukutholwa okusheshayo nokunembile kokuguqulwa kofuzo lwe-K-ras kubaluleke kakhulu ekuqondisweni kwemithi yomtholampilo.Le khithi isebenzisa i-DNA njengesampula yokutholwa ukuze inikeze ukuhlolwa kwekhwalithi kwesimo sokuguquka, okungasiza odokotela ekuhloleni umdlavuza we-colorectal, umdlavuza wamaphaphu nezinye iziguli zesimila ezihlomula emithini eqondiwe.Imiphumela yokuhlolwa yekhithi ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa komuntu ngamunye kweziguli.Odokotela kufanele benze izinqumo ezibanzi emiphumeleni yokuhlolwa ngokusekelwe ezicini ezifana nesimo sesiguli, izinkomba zemithi, ukusabela kokwelashwa nezinye izinkomba zokuhlola elabhorethri.

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni
Impilo yeshelufu Uketshezi: izinyanga ezingu-9;I-Lyophilized: izinyanga eziyi-12
Uhlobo lwesifanekiso izicubu ze-pathological ezishumekwe upharafini noma ingxenye iqukethe amaseli anesimila
CV ≤5.0%
LoD I-K-ras Reaction Buffer A kanye ne-K-ras Reaction Buffer B ingathola ngokuzinzile izinga lokuguqulwa elingu-1% ngaphansi kwengemuva elingu-3ng/μL lohlobo lwasendle
Izinsimbi Ezisebenzayo I-Applied Biosystems 7500 Real-Time PCR Systems

I-Applied Biosystems 7300 Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler® 480 Real-Time

I-BioRad CFX96 Real-Time PCR System

Ukugeleza komsebenzi

Kunconywa ukusebenzisa i-QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) kanye ne-Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) ekhiqizwa i-Tiangen Biotech(Beijing) Co., Ltd.


  • Okwedlule:
  • Olandelayo:

  • Bhala umlayezo wakho lapha futhi usithumelele wona