I-ROS1 Fusion Gene Mutation yabantu

Incazelo emfushane:

Le kit isetshenziselwa ukutholwa kwekhwalithi ye-in vitro yezinhlobo eziyi-14 zokuguqulwa kofuzo lwe-ROS1 kumasampula omdlavuza wamaphaphu wamangqamuzana angewona amancanyana (Ithebula 1).Imiphumela yokuhlolwa ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa komuntu ngamunye kweziguli.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-TM009-Human ROS1 Fusion Gene Mutation Detection Kit (Fluorescence PCR)

Isitifiketi

CE

I-Epidemiology

I-ROS1 iyi-transmembrane tyrosine kinase yomndeni wama-insulin receptor.I-ROS1 fusion gene iye yaqinisekiswa njengolunye ufuzo olubalulekile olungelona oluncane lomshayeli womdlavuza wamaphaphu.Njengommeleli wohlobo oluncane lwamangqamuzana olusha oluhlukile, isigameko sofuzo oluhlanganisiwe lwe-ROS1 ku-NSCLC Cishe u-1% kuya ku-2% we-ROS1 uhlelwa kabusha yisakhi sofuzo kuma-exons ayo angu-32, 34, 35 kanye no-36. Ngemva kokuba ehlanganiswe nezakhi zofuzo ezifana ne-CD74, I-EZR, SLC34A2, ne-SDC4, izoqhubeka nokusebenzisa isifunda se-ROS1 tyrosine kinase.I-ROS1 kinase ecushwe ngokungavamile ingavula izindlela zokusayina ezansi nomfula njenge-RAS/MAPK/ERK, PI3K/Akt/mTOR, kanye ne-JAK3/STAT3, ngaleyo ndlela ibambe iqhaza ekwandeni, ukuhlukaniswa kanye nokumetastase kwamaseli wesimila, nokudanga umdlavuza.Phakathi kwezinguquko zokuhlanganisa i-ROS1, i-CD74-ROS1 ithatha cishe u-42%, i-EZR yenza cishe u-15%, i-SLC34A2 yenza cishe u-12%, kanti i-SDC4 yenza cishe u-7%.Ucwaningo luye lwabonisa ukuthi isayithi elibophezela i-ATP lesizinda se-catalytic se-ROS1 kinase kanye nesayithi elibopha i-ATP le-ALK kinase line-homology efika ku-77%, ngakho-ke i-ALK tyrosine kinase i-molecule encane inhibitor crizotinib nokunye kunomphumela osobala wokwelapha. ekwelapheni i-NSCLC ngokuguqulwa kwe-fusion ye-ROS1.Ngakho-ke, ukutholwa kokuguqulwa kokuhlanganiswa kwe-ROS1 kuyisisekelo nesisekelo sokuqondisa ukusetshenziswa kwezidakamizwa ze-crizotinib.

Isiteshi

FAM Ibhafa yokusabela 1, 2, 3 kanye no-4
I-VIC(HEX) Isivimbeli sokusabela 4

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃

Impilo yeshelufu

9 izinyanga

Uhlobo lwesifanekiso

izicubu ze-pathological ezishumekwe upharafini noma amasampula asikiwe

CV

<5.0%

Ct

≤38

LoD

Le khithi ingathola ukuguqulwa kokuhlanganiswa okuphansi okungamakhophi angama-20.

Izinsimbi Ezisebenzayo:

I-Applied Biosystems 7500 Real-Time PCR SystemsApplied Biosystems 7500 Fast Real-Time PCR Systems

I-SLAN ®-96P Real-Time PCR Systems

I-QuantStudio™ 5 Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-RNeasy FFPE Kit (73504) evela ku-QIAGEN, Isigaba Sezicubu Ezishumekiwe Upharafini Isamba Sekhithi Yokukhipha I-RNA(DP439) evela e-Tiangen Biotech(Beijing) Co.,Ltd.


  • Okwedlule:
  • Olandelayo:

  • Bhala umlayezo wakho lapha futhi usithumelele wona