I-Methylated Yomuntu NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene

Incazelo emfushane:

Ikhithi ihloselwe ukutholwa kwekhwalithi ye-in vitro yezakhi zofuzo ze-methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 kumaseli akhishwe emathunjini kumasampula endle yomuntu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-OT077-Human Methylated NDRG4/SEPT9/SFRP2/BMP3/SDC2 Gene Detection Kit (Fluorescence PCR)

Isitifiketi

CE

I-Epidemiology

Kubantu abadala, amaseli e-epithelial amathumbu angaphezu kuka-10 8 awela odongeni lwamathumbu nsuku zonke, futhi akhishwa ngendle ngokusebenzisa i-peristalsis yamathumbu amakhulu.Ngenxa yamangqamuzana e-tumor maningi amathuba okuthi awele emgudwini wamathumbu wokwanda okungavamile, isihlalo seziguli zesimila samathumbu siqukethe amaseli amaningi anesifo kanye nezingxenye zamangqamuzana ezingavamile, okuyisisekelo esibonakalayo sokutholwa kwendle ezinzile.Ucwaningo luthole ukuthi ukuguqulwa kwe-methylation yabagqugquzeli bezakhi zofuzo kuyisenzakalo sakuqala ku-tumorigenesis, futhi izinto zofuzo ezitholakala kumasampula esitulo eziguli ezinomdlavuza we-colorectal zingabonisa ukuba khona komdlavuza emathunjini ngaphambili.

I-NDRG4, eyaziwa nangokuthi i-SMAP-8 kanye ne-BDM1, ingelinye lamalungu amane omndeni wofuzo we-NDRG (NDRG1-4), okuye kwaboniswa ukuthi kuhlotshaniswa nokwanda kwamaseli, ukuhlukaniswa, ukuthuthukiswa kanye nokucindezeleka.Kuyaqinisekiswa ukuthi i-NDRG4 methylation iyi-biomarker engaba khona yokutholwa okungahlaseli komdlavuza we-colorectal kumasampula esitulo.

U-SEPT9 uyilungu lomndeni we-Septin gene, equkethe okungenani izakhi zofuzo eziyi-13 ezifaka ikhodi yesizinda se-GTPase esilondoloziwe esingabopha amaprotheni ahlobene ne-cytoskeleton, futhi ehlotshaniswa nokuhlukaniswa kwamaseli kanye ne-tumorigenesis.Ucwaningo luthole ukuthi okuqukethwe kofuzo lwe-methylated Septin9 kunyuswa ngokuphawulekayo kumasampula esitulo ezigulini ezinomdlavuza we-colorectal.

Amaprotheni ayimfihlo ahlobene ne-frizzled (sFRPs) angamaprotheni ancibilikayo ayisigaba sabaphikisi bendlela ye-Wnt ngenxa ye-homology yabo yesakhiwo ephezulu kuma-frizzled (Fz) receptor for Wnt signing.Ukungasebenzi kofuzo lwe-SFRP kuphumela ekusebenzeni okungalawulwa kokusayina kwe-Wnt okuhlobene nomdlavuza we-colorectal.Njengamanje, i-SFRP2 methylation esitokisini ingasetshenziswa njenge-biomarker engahlaseli ekuxilongeni umdlavuza we-colorectal.

I-BMP3 iyilungu lomndeni omkhulu we-TGF-B futhi ngaleyo ndlela idlala indima ebalulekile ekukhuleni kombungu ngokunxenxa kanye nokubumba ukwakheka kwamathambo okuqala.I-BMP3 i-hypermethylated kumdlavuza we-colorectal futhi ingasetshenziswa njengomaka obalulekile wesimila.

I-SDC2 iyindawo yeseli ye-heparan sulfate proteoglycan ebandakanyeka ekulawuleni izinqubo eziningi ze-physiological and pathological.Ukucutshungulwa ngokomzimba kuhlanganisa ukwanda kwamaseli, ukuhlukaniswa, ukunamathela, inhlangano ye-cytoskeletal, ukufuduka, ukuphulukiswa kwesilonda, ukuxhumana kwe-cell-matrix, i-angiogenesis;Izinqubo ze-Pathological zihlanganisa ukuvuvukala nomdlavuza.Izinga le-methylation lofuzo lwe-SDC2 ezicutshini zomdlavuza we-colorectal laliphezulu kakhulu kunalelo lezicubu ezivamile.

Isiteshi

Isilondolozi sokusabela A

VIC/HEX i-methylated NDRG4 gene
I-ROX i-methylated SEPT9 gene
CY5 ukulawula kwangaphakathi

Isilondolozi sokusabela B

VIC/HEX i-methylated SFRP2 yofuzo
I-ROX i-methylated BMP3 gene
FAM i-methylated SDC2 gene
CY5 ukulawula kwangaphakathi

Ukuhunyushwa

Gene

Isiteshi Sesiginali

Ct Inani

Ukuhunyushwa

I-NDRG4

I-VIC (HEX)

Inani le-CT≤38

I-NDRG4 enhle

Ct value>38 noma ngaphansi

I-NDRG4 negative

SEPT9

I-ROX

Inani le-CT≤38

U-SEPT9 omuhle

Ct value>38 noma ngaphansi

SEPT9 negative

I-SFRP2

I-VIC (HEX)

Inani le-CT≤38

I-SFRP2 enhle

Ct value>38 noma ngaphansi

I-SFRP2 inegethivu

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃
Impilo yeshelufu 9 izinyanga
Uhlobo lwesifanekiso Isampula yendle
CV ≤5.0%
Ukucaciswa Akukho ukusabela okuphambene nomdlavuza wesibindi, umdlavuza we-bile duct, umdlavuza wegilo kanye nomdlavuza wamaphaphu
Izinsimbi Ezisebenzayo I-QuantStudio ®5 I-Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

I-MA-6000 Real-Time Quantitative Thermal Cycler

Ukugeleza komsebenzi

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit(HWTS-3001, HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS- 3006).


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