Human BRAF Gene V600E Ukuguqulwa

Incazelo emfushane:

Le khithi yokuhlola isetshenziselwa ukuthola ngokufanelekile ukuguqulwa kofuzo lwe-BRAF lwe-V600E kumasampula ezicubu ezishunyekiwe zikapharafini zemelanoma yomuntu, umdlavuza we-colorectal, umdlavuza wegilo kanye nomdlavuza wamaphaphu in vitro.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-TM007-Human BRAF Gene V600E Mutation Detection Kit(Fluorescence PCR)

Isitifiketi

CE

I-Epidemiology

Zingaphezu kwama-30 izinhlobo zokuguqulwa kwe-BRAF ezitholakele, cishe u-90% wazo zitholakala ku-exon 15, lapho ukuguqulwa kwe-V600E kuthathwa njengokujwayelekile kakhulu, okungukuthi, i-thymine(T) esendaweni 1799 ku-exon 15 iguqulelwe ku- i-adenine (A), okuholela ekushintshweni kwe-valine (V) endaweni engu-600 nge-glutamic acid (E) emkhiqizweni wamaprotheni.Ukuguqulwa kwe-BRAF kuvame ukutholakala ezimila eziyingozi njengemelanoma, umdlavuza we-colorectal, umdlavuza wegilo, nomdlavuza wamaphaphu.Ukuqonda ukuguqulwa kofuzo lwe-BRAF sekuyisidingo sokuhlola izidakamizwa ze-EGFR-TKIs kanye ne-BRAF eqondiswe kokuguqulwa kofuzo ekwelashweni kwezidakamizwa okuhlosiwe ezigulini ezingazuza.

Isiteshi

FAM Ukuguqulwa kwe-V600E, ukulawula kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃

Impilo yeshelufu

9 izinyanga

Uhlobo lwesifanekiso

amasampula ezicubu ze-pathological afakwe upharafini

CV

<5.0%

Ct

≤38

LoD

Sebenzisa amakhithi ukuthola ukulawulwa kwekhwalithi okuhambisanayo kwe-LoD.a) ngaphansi kuka-3ng/μL ingemuva lohlobo lwasendle, izinga lokuguqulwa elingu-1% lingatholwa kusigcinalwazi sokusabela ngokuzinzile;b) ngaphansi kwesilinganiso esingu-1% sokuguqulwa, ukuguqulwa kwe-1 × 103Amakhophi/mL kungemuva lohlobo lwasendle lwe-1×105Amakhophi/mL angatholwa ngokuzinza kusigcinalwazi sokusabela;c) I-IC Reaction Buffer ingathola umkhawulo wokuthola umkhawulo ophansi wokulawula ikhwalithi SW3 wokulawula kwangaphakathi kwenkampani.

Izinsimbi Ezisebenzayo:

I-Applied Biosystems 7500 Real-Time PCR SystemsI-Applied Biosystems 7300 Real-Time PCR

Amasistimu, I-QuantStudio® 5 Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-BioRad CFX96 Real-Time PCR System

Ukugeleza komsebenzi

Ama-reagents okukhipha anconyiwe: I-QIAGEN's QIAamp DNA FFPE Tissue Kit (56404), I-Tissue DNA Rapid Extraction Kit eshumekwe Upharafini (DP330) ekhiqizwe i-Tiangen Biotech(Beijing) Co., Ltd.


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