I-Coxsackie Virus Uhlobo lwe-A16 Nucleic Acid

Incazelo emfushane:

Le kit isetshenziselwa ukuthola ikhwalithi ye-in vitro yegciwane le-Coxsackie i-A16 nucleic acid kumaswabhu omphimbo womuntu.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

I-HWTS-EV025-Coxsackie Virus Type A16 Nucleic Acid Detection Kit (Enzymatic Probe Isothermal Amplification)

Isitifiketi

CE

I-Epidemiology

Le kit yamukela i-Enzymatic Probe Isothermal Amplification (EPIA), futhi yakha ama-primers athile kanye ne-RNA base-containing probes (rProbe) yesifunda esilondolozwe kakhulu se-Cox A16, futhi yengeza i-Bst enzyme kanye ne-enzyme ye-RNaseH ngesikhathi esifanayo, lapho kwesokunxele kanye iziphetho ezingakwesokudla zesisekelo se-RNA se-rProbe zilebulwe ngamaqembu e-fluorescent nesicimi, ngokulandelanayo.Sebenzisa umsebenzi we-DNA polymerase kanye nomsebenzi wokususwa kwe-strand we-enzyme ye-Bst ukuze ukhulise ithagethi ukuze ihlolwe ezingeni lokushisa elingaguquki, i-enzyme ye-RNaseH ingahlukanisa izisekelo ze-RNA kuchungechunge lwe-hybrid-probe hybrid, ukuze iqembu le-fluorescent kanye nesicimi se-rProbe kube. ihlukaniswe ngaleyo ndlela i-fluorescing.Ngaphezu kwalokho, ucezu lwesokunxele lwesisekelo se-rProbe RNA esisele singasetshenziswa njengesiqalo ukuze sinwetshwe ukuze sakhe umkhiqizo, ophinde uqongelele umkhiqizo.Isignali ye-fluorescent iqoqwa ngokuqhubekayo ngokwakhiwa komkhiqizo, ngaleyo ndlela ibona ukutholwa kwe-nucleic acid ehlosiwe.

Isiteshi

FAM I-Coxsackie virus uhlobo A16
I-ROX I-contro yangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃

Impilo yeshelufu

Izinyanga ezingu-12

Uhlobo lwesifanekiso

Ama-swabs omphimbo asanda kuqoqwa

CV

≤10.0%

Ct

≤38

LoD

2000Amakhophi/mL

Izinsimbi Ezisebenzayo

I-Applied Biosystems 7500 Real-Time PCR SystemsSLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

I-Real-time Fluorescence Constant Temperature System Easy Amp HWTS1600

Ukugeleza komsebenzi

Inketho 1

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006)

Inketho yesi-2

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Sample Release Reagent (HWTS-3005-8).


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