Izinhlobo eziyi-15 ze-Human Papillomavirus E6/E7 Gene mRNA

Incazelo emfushane:

Le khithi ihloselwe ukuthola amazinga angu-15 anobungozi obukhulu be-human papillomavirus (HPV) E6/E7 gene mRNA kumaseli akhishiwe omlomo wesibeletho wesifazane.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

HWTS-CC005A-15 Izinhlobo ze-Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR)

I-Epidemiology

Umdlavuza womlomo wesibeletho ungolunye lwezinhlobo ezivame kakhulu zomdlavuza wabesifazane emhlabeni wonke, futhi ukwenzeka kwawo kuhlobene eduze nama-human papillomaviruses (HPV), kodwa ingxenye encane kuphela yokutheleleka nge-HPV engakhula ibe umdlavuza.I-HPV enobungozi obukhulu ihlasela amaseli e-epithelial yomlomo wesibeletho futhi ikhiqize ama-oncoprotein amabili, i-E6 ne-E7.Le phrotheni ingathinta izinhlobonhlobo zamaphrotheni eselula (njengamaphrotheni e-tumor suppressor pRB kanye ne-p53), andise umjikelezo weseli, ithinte ukuhlanganiswa kwe-DNA nokuzinza kwe-genome, futhi iphazamise ukusabela kwe-antiviral kanye ne-antitumor immune.

Isiteshi

Isiteshi Isakhi I-Genotype ihloliwe
FAM I-HPV Reaction Buffer 1 HPV16,31,33,35,51,52,58
VIC/HEX Isakhi sofuzo se-β-actin yomuntu
FAM I-HPV Reaction Buffer 2 I-HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX Isakhi sofuzo somuntu we-INS

Imingcele Yezobuchwepheshe

Isitoreji Uketshezi: ≤-18℃
Impilo yeshelufu 9 izinyanga
Uhlobo lwesifanekiso iseli yomlomo wesibeletho exfoliated
Ct ≤38
CV ≤5.0%
LoD 500 Amakhophi/mL
Izinsimbi Ezisebenzayo I-Applied Biosystems 7500 Real-Time PCR System

Applied Biosystems 7500 Fast Real-Time PCR Systems

I-QuantStudio®5 Real-Time PCR Systems

SLAN-96P Real-Time PCR Systems

Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time

LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola

I-MA-6000 Real-Time Quantitative Thermal Cycler

I-BioRad CFX96 Real-Time PCR System

I-BioRad CFX Opus 96 Real-Time PCR System

Ukugeleza komsebenzi

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) yi-Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ukukhishwa kufanele kwenziwe ngokulandela imiyalelo yokusetshenziswa. .Ivolumu ye-elution enconyiwe ngu-50μL.Uma isampula lingagayiwe ngokuphelele, libuyisele esinyathelweni sesi-4 ukuze ligaywe kabusha.Bese uvivinya ngokwemiyalelo yokusetshenziswa.

Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431).Ukukhishwa kufanele kwenziwe ngokwemiyalelo yokusetshenziswa ngokuqinile (Esinyathelweni sesi-5, phinda kabili ukugxiliswa kwesisombululo sokusebenza se-DNaseI, okungukuthi, thatha u-20μL wesisombululo sesitoko se-RNase-Free DNaseI (1500U) uwafake eshubhuni entsha ye-RNase-Free centrifuge, engeza u-60μL webhafa ye-RDD, bese uxuba ngobumnene).Ivolumu ye-elution enconyiwe ngu-60μL.Uma isampula lingagayiwe ngokuphelele, libuyisele esinyathelweni sesi-5 ukuze ligaywe kabusha.Bese uvivinya ngokwemiyalelo yokusetshenziswa.


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