I-Staphylococcus Aureus kanye ne-Methicillin-Resistant Staphylococcus Aureus Nucleic Acid
Igama lomkhiqizo
I-HWTS-OT062-Staphylococcus Aureus kanye ne-Methicillin-Resistant Staphylococcus Aureus Nucleic Acid Detection Kit (Fluorescence PCR)
Isitifiketi
CE
I-Epidemiology
I-Staphylococcus aureus ingenye yebhaktheriya ebalulekile ye-pathogenic ye-nosocomial infection.I-Staphylococcus aureus (SA) ingeye-staphylococcus futhi imele amagciwane e-Gram-positive, angakhiqiza ubuthi obuhlukahlukene nama-enzyme ahlaselayo.Amabhaktheriya anezici zokusabalalisa okubanzi, i-pathogenicity eqinile kanye nezinga eliphezulu lokumelana.I-nuclease gene ye-thermostable (i-nuc) iwufuzo olugcinwe kakhulu lwe-staphylococcus aureus.Eminyakeni yamuva nje, ngenxa yokusetshenziswa kakhulu kwama-hormone namalungiselelo okuzivikela komzimba kanye nokusetshenziswa kabi kwemithi elwa namagciwane ebanzi, izifo ze-nosocomial ezibangelwa i-Methicillin-resistant Staphylococcus aureus (MRSA) eStaphylococcus ziye zanda.Izinga lokutholwa elimaphakathi likazwelonke le-MRSA lalingu-30.2% ngo-2019 e-China.I-MRSA ihlukaniswe yaba yi-MRSA (HA-MRSA) ehambisana nomphakathi, i-MRSA ehlotshaniswa nomphakathi (CA-MRSA), kanye ne-MRSA ehlobene nemfuyo (LA-MRSA).I-CA-MRSA, HA-MRSA, LA-MRSA inomehluko omkhulu ku-microbiology, ukumelana namagciwane (isb, i-HA-MRSA ikhombisa ukumelana nezidakamizwa eziningi kune-CA-MRSA) kanye nezici zomtholampilo (isb indawo yokutheleleka).Ngokwalezi zici, i-CA-MRSA ne-HA-MRSA ingahlukaniswa.Kodwa-ke, umehluko phakathi kwe-CA-MRSA ne-HA-MRSA uyancipha ngenxa yokuhamba njalo kwabantu phakathi kwezibhedlela nemiphakathi.I-MRSA imelana nemithi eminingi, ayizweli kuphela kuma-antibiotic e-β-lactam, kodwa futhi kuma-aminoglycosides, ama-macrolides, ama-tetracycline nama-quinolones ngamazinga ahlukahlukene.Kunomehluko omkhulu wesifunda emazingeni okumelana nezidakamizwa kanye nezitayela ezahlukene.
I-Methicillin ukumelana ne-mecA yofuzo idlala indima ewujuqu ekumelana ne-staphylococcal.Isakhi sofuzo sithwala isakhi sofuzo esiyingqayizivele seselula (i-SCCmec), esifaka iphrotheni engu-2a ebopha i-penicillin (PBP2a) futhi inobudlelwano obuphansi nama-antibiotic e-β-lactam, ukuze izidakamizwa ezilwa namagciwane zingakwazi ukuvimbela ukwakheka kongqimba lweseli lwe-peptidoglycan, okuholela ekuphikisweni kwezidakamizwa.
Isiteshi
| FAM | Ufuzo lwe-mecA olumelana ne-methicillin |
| CY5 | i-staphylococcus aureus nuc gene |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | amasampula okutheleleka ngesikhwehlela, isikhumba nezicubu ezithambile, namasampula egazi eliphelele |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL |
| Ukucaciswa | Akukho-reactivity nezinye izifo zokuphefumula ezifana ne-methicillin-sensitive staphylococcus aureus, i-coagulase-negative staphylococcus, i-methicillin-resistant staphylococcus epidermidis, i-pseudomonas aeruginosa, i-escherichia coli, i-klebsicineus pneumoniae, i-asteococcus, i-klebsimaninobamiraeter, i-klebsimanobaicoccus, i-klebsimanyococcus, i-prophylococcus ye-methicillin. i-cter cloacae, i-streptococcus pneumoniae , enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
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