I-SARS-CoV-2 influenza A umkhuhlane B Nucleic Acid Ihlanganiswe
Igama lomkhiqizo
HWTS-RT060A-SARS-CoV-2 umkhuhlane A umkhuhlane B Nucleic Acid Combined Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Corona Virus Disease 2019 (COVID-19) ibangelwa yi-SARS-CoV-2 okungeye-β Coronavirus yohlobo.I-COVID-19 yisifo esithathelwanayo esinamandla sokuphefumula, futhi isixuku ngokuvamile siyathinteka.Njengamanje, iziguli ezinegciwane le-SARS-CoV-2 ziwumthombo oyinhloko wokutheleleka, futhi iziguli ezingenazimpawu zingase zibe umthombo wokutheleleka.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezingu-1-14, ikakhulukazi izinsuku ezingu-3-7.Ukubonakaliswa okuyinhloko kwakuwumkhuhlane, ukukhwehlela okomile nokukhathala.Iziguli ezimbalwa zinezimpawu ezinjengokuminyana emakhaleni, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo.
I-Influenza yisifo esiyingozi sokuphefumula esibangelwa igciwane lomkhuhlane.Ithathelwana kakhulu futhi isakazeka kakhulu ngokukhwehlela kanye nokuthimula.Ivamise ukuqubuka entwasahlobo nasebusika.Kunezinhlobo ezintathu zoMkhuhlane, Umkhuhlane A (IFV A), Umkhuhlane B (IFV B) kanye Nomkhuhlane C (IFV C), zombili zingezomndeni we-ortomyxovirus.I-Influenza A kanye no-B, okungamagciwane e-RNA ane-stranded eyodwa, yizimbangela eziyinhloko zezifo zabantu.Umkhuhlane A yisifo esithathelanayo esinamandla sokuphefumula, okuhlanganisa i-H1N1, i-H3N2 nezinye izinhlobo ezincane, kulula ukusishintsha.Ukuqubuka kwembulunga yonke, "ukushintsha" kubhekise ekuguquguqukeni komkhuhlane A, okuholela "kuhlobo oluncane" lwegciwane elisha.Umkhuhlane B uhlukaniswe izigaba ezimbili: i-Yamagata ne-Victoria.Umkhuhlane B une-antigenic drift kuphela, futhi ugwema ukubhekwa nokuqedwa amasosha omzimba womuntu ngokuguqula izakhi.Kodwa amagciwane omkhuhlane B ashintsha kancane kakhulu kunomkhuhlane womuntu A, nawo obangela izifo zokuphefumula kanye nobhadane kubantu.
Isiteshi
| FAM | I-SARS-CoV-2 |
| I-ROX | IFV B |
| CY5 | IFV A |
| I-VIC(HEX) | Izakhi zofuzo zokulawula kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
| I-Lyophilization: ≤30℃ ebumnyameni | |
| Impilo yeshelufu | Uketshezi: izinyanga ezingu-9 |
| I-Lyophilization: izinyanga eziyi-12 | |
| Uhlobo lwesifanekiso | I-Nasopharyngeal swabs, i-Oropharyngeal swabs |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 300 Amakhophi/mL |
| Ukucaciswa | Imiphumela yokuhlolwa kwesiphambano ibonise ukuthi ikhithi iyahambisana ne-coronavirus yabantu i-SARSr- CoV, MERSr-CoV, HcoV-OC43, HcoV-229E, HcoV-HKU1, HCoV-NL63, i-respiratory syncytial virus A no-B, parainfluenza virus 1, 2 kanye 3, i-rhinovirusA, B no-C, i-adenovirus 1, 2, 3, 4, 5, 7 kanye no-55, i-metapneumovirus yabantu, i-enterovirus A, B, C no-D, igciwane le-cytoplasmic pulmonary virus, igciwane le-EB, igciwane lesimungumungwane I-cytomegalovirus yabantu, i-rotavirus, i-norovirus, igciwane le-mumps, i-varicella zoster virus, i-Mycoplasma pneumoniae, i-Chlamydia pneumoniae, i-Legionella, i-pertussis, i-Haemophilus influenzae, i-Staphylococcus aureus, i-Streptococcus pneumoniae, i-Streptococcus pyogenes, i-Klebsiella pneumoniae, i-Klebsiella pneumoniae, i-Mycobacterium fuvulcandidas, i-Caspylococcus aureus kwakungekho ukusabela okuphambene phakathi kwe-Pneumocystis yersini kanye ne-Cryptococcus neoformans. |
| Izinsimbi Ezisebenzayo: | Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emakethe. I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: Isizinda se-Nucleic Acid noma i-Purification Reagent(YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.
