Amagciwane Okuphefumula Ahlanganisiwe
Igama lomkhiqizo
I-HWTS-RT106A-Ikhithi Yokuthola Amagciwane Ahlanganisiwe Yokuphefumula (i-Fluorescence PCR)
I-Epidemiology
Izifo ezibangelwa ama-microorganisms e-pathogenic ahlasela ikhala lomuntu, umphimbo, uqhoqhoqho, i-bronchus, amaphaphu nezinye izicubu zokuphefumula nezitho futhi anda zibizwa ngokuthi izifo zepheshana lokuphefumula.Izimpawu zomtholampilo zihlanganisa imfiva, ukukhwehlela, ikhala eligijimayo, umphimbo obuhlungu, ukukhathala okuvamile nokuba buhlungu .Amagciwane okuphefumula ahlanganisa amagciwane, i-mycoplasma, i-chlamydia, amagciwane, njll. Iningi lezifo libangelwa amagciwane.Amagciwane okuphefumula anezinhlamvu ezilandelayo njengezinhlobo eziningi zezinhlobo, ukuziphendukela kwemvelo okusheshayo, ama-subtypes ayinkimbinkimbi, izimpawu ezifanayo zomtholampilo.Inezinhlamvu zomtholampilo ezifana nokuqala okusheshayo, ukusabalala okusheshayo, ukusuleleka okuqinile, nezimpawu ezifanayo okunzima ukuzihlukanisa, ezibeka usongo olukhulu empilweni yomuntu.
Isiteshi
FAM | IFV A, IFV B Victoria, PIV uhlobo 1, hMPV uhlobo 2, ADV, RSV uhlobo A, MV· |
I-VIC(HEX) | IFV B, H1, IFV B Yamagata, inkomba yangaphakathi |
CY5 | Ireferensi yangaphakathi, uhlobo lwe-PIV 3, uhlobo lwe-hMPV1, uhlobo lwe-RSV B |
I-ROX | Ireferensi yangaphakathi, H3, PIV uhlobo 2 |
Imingcele Yezobuchwepheshe
Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
Impilo yeshelufu | 9 izinyanga |
Uhlobo lwesifanekiso | Ama-swabs e-oropharyngeal asanda kuqoqwa |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 500Amakhophi/mL |
Ukucaciswa | Akukho ukuhlangana kabusha nge-genome yomuntu namanye amagciwane okuphefumula. |
Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emakethe. Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |