I-Mycobacterium Tuberculosis Nucleic Acid kanye ne-Rifampicin Resistance

Incazelo emfushane:

Le khithi ifanele ukutholwa kwekhwalithi ye-Mycobacterium tuberculosis DNA kumasampula esikhwehlela somuntu ku-vitro, kanye nokuguqulwa kwe-homozygous endaweni engu-507-533 ye-amino acid ye-codon yofuzo lwe-rpoB ebangela ukumelana ne-Mycobacterium tuberculosis rifampicin.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

HWTS-RT074B-Mycobacterium Tuberculosis Nucleic Acid and Rifampicin Resistance Detection Kit (Melting Curve)

Isitifiketi

CE

I-Epidemiology

I-Mycobacterium tuberculosis , ngokushesha nje njenge-Tubercle bacillus, i-TB, igciwane le-pathogenic elidala isifo sofuba.Njengamanje, imithi elwa nesifo sofuba yomugqa wokuqala esetshenziswa kakhulu ihlanganisa i-isoniazid, i-rifampicin ne-hexambutol, njll. Imithi yomugqa wesibili yokulwa nesifo sofuba ihlanganisa i-fluoroquinolones, i-amikacin ne-kanamycin, njll. Imithi emisha ethuthukisiwe i-linezolid, i-bedaquiline ne-delamani, njll. Nokho, ngenxa yokusetshenziswa okungalungile kwemithi elwa nesifo sofuba kanye nezici zesakhiwo sodonga lwamangqamuzana e-mycobacterium tuberculosis, isifo sofuba se-mycobacterium siqala ukumelana nemithi elwa nesifo sofuba, okuletha izinselele ezinkulu ekuvimbeleni nasekwelapheni isifo sofuba.

I-Rifampicin isetshenziswe kabanzi ekwelapheni iziguli ezinesifo sofuba samaphaphu kusukela ngasekupheleni kwawo-1970, futhi inomphumela obalulekile.Kube yisinqumo sokuqala ukufushanisa ukwelashwa ngamakhemikhali ezigulini ze-pulmonary tuberculosis.Ukumelana ne-rifampicin kubangelwa ikakhulukazi ukuguqulwa kofuzo lwe-rpoB.Nakuba imishanguzo emisha yokulwa nesifo sofuba ihlale iphuma, kanye nokusebenza emtholampilo kweziguli ezinesifo sofuba samaphaphu nakho kuqhubekile nokuba ngcono, kusenokuntuleka kwezidakamizwa ezilwa nesifo sofuba, futhi ukwenzeka kokusetshenziswa kwezidakamizwa okungenangqondo emtholampilo kuphezulu uma kuqhathaniswa.Ngokusobala, isifo sofuba iMycobacterium ezigulini ezinesifo sofuba samaphaphu asikwazi ukubulawa ngokuphelele ngesikhathi esifanele, okuholela emazingeni ahlukene okumelana nemithi emzimbeni wesiguli, sandise isikhathi sokugula, futhi kwandisa ingozi yokufa kwesiguli.

Isiteshi

Isiteshi

Iziteshi kanye Fluorophores

Isivimbeli sokusabela A

Isivimbeli sokusabela B

Isivimbeli sokusabela C

Isiteshi se-FAM

Intatheli: FAM, Quencher: Akukho

I-rpoB 507-514

I-rpoB 513-520

38KD kanye ne-IS6110

Isiteshi se-CY5

Intatheli: CY5, Quencher: Akukho

I-rpoB 520-527

rpoB 527-533

/

Isiteshi se-HEX (VIC).

Intatheli: HEX (VIC), Quencher: Akukho

Ukulawula kwangaphakathi

Ukulawula kwangaphakathi

Ukulawula kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji

≤-18℃ ebumnyameni

Impilo yeshelufu

Izinyanga ezingu-12

Uhlobo lwesifanekiso

Isikhwehlela

CV

≤5.0%

LoD

i-mycobacterium tuberculosis 50 amagciwane/mL

uhlobo lwasendle olumelana ne-rifampicin: 2x103amagciwane/mL

i-homozygous mutant: 2x103amagciwane/mL

Ukucaciswa

Ithola uhlobo lwasendle lwe-mycobacterium tuberculosis kanye nezindawo eziguqukayo zezinye izakhi zofuzo ezimelana nezidakamizwa ezifana ne-katG 315G>C\A, InhA-15C> T, imiphumela yokuhlola ikhombisa ukungamelani ne-rifampicin, okusho ukuthi akukho ukusabela okuphambene.

Izinsimbi Ezisebenzayo:

SLAN-96P Real-Time PCR Systems

I-BioRad CFX96 Real-Time PCR System

I-LightCycler480® Real-Time PCR System

Ukugeleza komsebenzi

4697e0586927f02cf6939f68fc30ffc


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