I-Mycobacterium Tuberculosis I-Isoniazid Resistance Mutation

I-Mycobacterium Tuberculosis Isoniazid Resistance Mutation Isithombe Esifakiwe

Incazelo emfushane:

Le khithi ifanele ukutholwa kwekhwalithi yezindawo eziyinhloko zokuguqula amasampula esikhwehlela somuntu aqoqwe ezigulini ezine-Tubercle bacillus positive eziholela ekumelaneni ne-mycobacterium tuberculosis isoniazid: Isifunda se-InhA sokukhuthaza -15C>T, -8T>A, -8T>C;Isifunda somgqugquzeli we-AhpC -12C>T, -6G>A;ukuguqulwa kwe-homozygous kwe-KatG 315 codon 315G>A, 315G>C.

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Igama lomkhiqizo

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)

I-Epidemiology

I-Mycobacterium tuberculosis, kafushane nje njenge-Tubercle bacillus (TB), igciwane le-pathogenic elidala isifo sofuba.Njengamanje, imithi elwa nesifo sofuba yomugqa wokuqala esetshenziswa kakhulu ihlanganisa i-isoniazid, i-rifampicin ne-hexambutol, njll. Imithi yomugqa wesibili yokulwa nesifo sofuba ihlanganisa i-fluoroquinolones, i-amikacin ne-kanamycin, njll. Imithi emisha ethuthukisiwe i-linezolid, i-bedaquiline ne-delamani, njll. Nokho, ngenxa yokusetshenziswa okungalungile kwemithi elwa nesifo sofuba kanye nezici zesakhiwo sodonga lwamangqamuzana e-mycobacterium tuberculosis, isifo sofuba se-mycobacterium siqala ukumelana nemithi elwa nesifo sofuba, okuletha izinselele ezinkulu ekuvimbeleni nasekwelapheni isifo sofuba.

Isiteshi

FAM	I-MP nucleic acid
I-ROX	Ukulawula Kwangaphakathi

Imingcele Yezobuchwepheshe

Isitoreji	≤-18℃
Impilo yeshelufu	Izinyanga ezingu-12
Uhlobo lwesifanekiso	isikhwehlela
CV	≤5.0%
LoD	Umkhawulo wokutholwa kohlobo lwasendle lwamagciwane amelana ne-isoniazid ngu-2x103 bacteria/mL, futhi umkhawulo wokutholwa kwamagciwane aguqukile ngu-2x103 bacteria/mL.
Ukucaciswa	a.Akukho ukusabela okuphambene phakathi kofuzo lomuntu, amanye amagciwane angenayo i-mycobacteria kanye ne-pneumonia atholwe yile khithi. b.Izindawo eziguqukayo zezinye izakhi zofuzo ezimelana nomuthi ohlotsheni lwasendle lwe-Mycobacterium tuberculosis, njengesifunda esinquma ukumelana kofuzo lwe-rifampicin rpoB, zitholiwe, futhi imiphumela yokuhlolwa ayizange ibonise ukumelana ne-isoniazid, okubonisa ukuthi akukho ukuphinda kusebenze okuphambene.
Izinsimbi Ezisebenzayo	SLAN-96P Real-Time PCR Systems I-BioRad CFX96 Real-Time PCR Systems I-LightCycler480® Real-Time PCR System

Ukugeleza komsebenzi

Uma usebenzisa i-Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (engasetshenziswa ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) nge-Jiangsu Macro & Micro-Test I-Med-Tech Co., Ltd. ukuze ikhishwe, engeza ama-200μL yokulawula okunegethivu kanye nesampula yesikhwehlela esicutshunguliwe ukuze sihlolwe ngokulandelana, bese wengeza u-10μL yokulawula kwangaphakathi ngokwehlukana ibe isilawuli esinegethivu, isampula yesikhwehlela esisetshenziwe ukuze sihlolwe, kanye nezinyathelo ezilandelayo kufanele zenziwe ngokuqinile ngokwemiyalelo yokukhipha.Umthamo wesampula okhishiwe ungama-200μL, futhi ivolumu ye-elution enconyiwe ingu-100μL.