I-Mycobacterium Tuberculosis INH Ukumelana
Igama Lomkhiqizo
HWTS-RT002A-Mycobacterium Tuberculosis Isoniazid Resistance Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Isoniazid, umuthi obalulekile wokulwa nesifo sofuba owethulwa ngo-1952, ungomunye wemishanguzo esebenza ngempumelelo kakhulu yokwelapha okuhlangene kwesifo sofuba esisebenzayo kanye nomuthi owodwa wesifo sofuba esicashile.
I-KatG iyisakhi sofuzo esiyinhloko esifaka ikhodi ye-catalase-peroxidase futhi ukuguqulwa kofuzo kwe-katG kungakhuthaza ukwakheka kodonga lwamaseli e-mycolic acid, kwenze amagciwane amelane ne-isoniazid.Isisho se-KatG sihlotshaniswa kabi nezinguquko ku-INH-MIC, futhi ukwehla okuphindwe izikhathi ezingu-2 kusisho se-katG kuphumela ekukhuphukeni okukhudlwana okuphindwe ka-2 ku-MIC.Enye imbangela yokumelana ne-isoniazid ku-mycobacterium tuberculosis kwenzeka lapho ukufakwa kwesisekelo, ukususwa noma ukuguqulwa kwenzeka endaweni yofuzo ye-InhA ye-mycobacterium tuberculosis.
Isiteshi
| I-ROX | indawo ye-inhA (-15C>T)· |
| CY5 | isayithi le-katG (315G>C). |
| I-VIC (HEX) | IS6110 |
Imingcele Yezobuchwepheshe
| Isitoreji | ≤-18℃ ebumnyameni |
| Impilo yeshelufu | Izinyanga ezingu-12 |
| Uhlobo lwesifanekiso | Isikhwehlela |
| CV | ≤5.0% |
| LoD | 1 × 103amagciwane/mL |
| Ukucaciswa | Ukusebenza kabusha okungenasiphambano nokuguqulwa kwezindawo ezine ezimelana nezidakamizwa (511, 516, 526 kanye 531) zofuzo lwe-rpoB ngaphandle kwebanga lokutholwa lekhithi yokuthola. Izinsimbi Ezisebenzayo: I-Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
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