I-Leukocyte yomuntu i-Antigen B27 Nucleic Acid
Igama lomkhiqizo
I-HWTS-GE011A-Human Leukocyte Antigen B27 Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
I-Ankylosing spondylitis (AS) isifo sokuvuvukala esiqhubekayo esingapheli esihlasela umgogodla futhi singase sihlanganise amalunga e-sacroiliac kanye namalunga azungezile kuya kumazinga ahlukahlukene.Kuvezwe ukuthi i-AS ikhombisa ukuhlangana komndeni okusobala futhi ihlobene eduze ne-antigen ye-HLA-B27 yomuntu ye-leukocyte.Kubantu, zingaphezu kuka-70 izinhlobo ze-HLA-B27 subtypes ezitholiwe futhi zakhonjwa, futhi kuzo, i-HLA-B*2702, HLA-B*2704 kanye ne-HLA-B*2705 yizinhlobo ezivame kakhulu ezihlobene nalesi sifo.E-China, Singapore, Japan kanye nesifunda sase-Taiwan sase-China, uhlobo oluncane oluvame kakhulu lwe-HLA-B27 yi-HLA-B*2704, lubalelwa ku-54%, lulandelwe yi-HLA-B*2705, ehlanganisa cishe u-41%.Le khithi ingathola i-DNA ezinhlotsheni ezincane ezithi HLA-B*2702, HLA-B*2704 kanye ne-HLA-B*2705, kodwa ayizihlukanisi kwezinye.
Isiteshi
| FAM | I-HLA-B27 |
| VIC/HEX | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
| Impilo yeshelufu | Uketshezi: izinyanga ezingu-18 |
| Uhlobo lwesifanekiso | amasampula egazi lonke |
| Ct | ≤40 |
| CV | ≤5.0% |
| LoD | 1ng/μL |
| Ukucaciswa
| Imiphumela yokuhlolwa etholwe yile khithi ngeke ithintwe i-hemoglobin (<800g/L), i-bilirubin (<700μmol/L), kanye ne-blood lipids/ triglycerides (<7mmol/L) egazini. |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-Applied Biosystems StepOne Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time I-Agilent-Stratagene Mx3000P Q-PCR System |
