Ukuguqulwa kwe-EGFR Yomuntu 29
Igama lomkhiqizo
I-HWTS-TM001A-EGFR I-Human EGFR Gene 29 Mutations Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza wamaphaphu usuyimbangela ehamba phambili yokufa komdlavuza emhlabeni wonke, obeka engcupheni impilo yabantu.Umdlavuza wamaphaphu ongewona omncane ubala cishe ama-80% eziguli ezinomdlavuza wamaphaphu.I-EGFR okwamanje iyinkomba ebaluleke kakhulu yamangqamuzana ekwelapheni umdlavuza wamaphaphu ongewona omncane.I-phosphorylation ye-EGFR ingakhuthaza ukukhula kwamangqamuzana e-tumor, ukuhlukaniswa, ukuhlasela, ukutholakala kwe-metastasis, i-anti-apoptosis, futhi ikhuthaze i-angiogenesis yesimila.I-EGFR tyrosine kinase inhibitors (TKI) ingavimba indlela yokusayina ye-EGFR ngokuvimbela i-EGFR autophosphorylation, ngaleyo ndlela ivimbele ukwanda nokuhlukaniswa kwamaseli wesimila, ikhuthaze i-tumor cell apoptosis, inciphise i-tumor angiogenesis, njll., ukuze kuzuzwe ukwelashwa okuhloswe kwesimila.Inani elikhulu locwaningo libonise ukuthi ukuphumelela kokwelapha kwe-EGFR-TKI kuhlobene eduze nesimo soguquko lwezakhi zofuzo ze-EGFR, futhi kungavimbela ngokuqondile ukukhula kwamaseli wesimila ngokuguqulwa kofuzo kwe-EGFR.Ufuzo lwe-EGFR lutholakala engalweni emfushane ye-chromosome 7 (7p12), enobude obugcwele obungu-200Kb futhi iqukethe ama-exons angu-28.Isifunda esiguquliwe sitholakala ikakhulukazi kuma-exons 18 kuya ku-21, ama-codons 746 kuya ku-753 asusa ukuguqulwa kuma-akhawunti e-exon 19 cishe ama-45% kanye nokuguqulwa kwe-L858R ku-exon 21 kubala cishe u-40% kuya ku-45%.Imihlahlandlela ye-NCCN Yokuxilongwa Nokwelashwa Komdlavuza Ongewona Owomncane Wengqamuzana Wamaphaphu isho ngokucacile ukuthi ukuhlolwa kokuguqulwa kofuzo kwe-EGFR kuyadingeka ngaphambi kokuphathwa kwe-EGFR-TKI.Le khithi yokuhlola isetshenziselwa ukuqondisa ukuphathwa kwezidakamizwa ze-epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), futhi inikeze isisekelo somuthi womuntu siqu ezigulini ezinomdlavuza wamaphaphu ongewona omncane.Le kit isetshenziselwa kuphela ukuthola ukuguqulwa okuvamile esakhini sofuzo se-EGFR ezigulini ezinomdlavuza wamaphaphu ongewona omncane.Imiphumela yokuhlolwa ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengesisekelo sodwa sokwelashwa komuntu ngamunye kweziguli.Odokotela kufanele bacabangele isimo sesiguli, izinkomba zomuthi, kanye nokwelashwa Ukusabela nezinye izinkomba zokuhlolwa kwaselabhorethri nezinye izici zisetshenziswa ukuze kwahlulele ngokuphelele imiphumela yokuhlolwa.
Isiteshi
| FAM | I-IC Reaction Buffer, L858R Reaction Buffer, 19del Rection Buffer, T790M Reaction Buffer, G719X Reaction Buffer, 3Ins20 Reaction Buffer, L861Q Reaction Buffer, S768I Reaction Buffer |
Imingcele Yezobuchwepheshe
| Isitoreji | Uketshezi: ≤-18℃ ebumnyameni;I-Lyophilized: ≤30℃ ebumnyameni |
| Impilo yeshelufu | Uketshezi: izinyanga ezingu-9;I-Lyophilized: izinyanga eziyi-12 |
| Uhlobo lwesifanekiso | izicubu zesimila esisha, isigaba esiqandisiwe se-pathological, izicubu ze-pathological ezishumekwe upharafini, i-plasma noma i-serum |
| CV | ＜5.0% |
| LoD | ukutholwa kwesixazululo se-nucleic acid ngaphansi kwesizinda sohlobo lwasendle elingu-3ng/μL, kungathola ngokuzinzile izinga lokuguqulwa elingu-1% |
| Ukucaciswa | Akukho ukuphinda kusebenze kabusha nge-DNA yomuntu yohlobo lwasendle nezinye izinhlobo eziguqukayo |
| Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR SystemsI-Applied Biosystems 7300 Real-Time PCR Systems I-QuantStudio® 5 Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler® 480 Real-Time I-BioRad CFX96 Real-Time PCR System |
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