I-Herpes Simplex Virus Type 1/2,(HSV1/2) Nucleic Acid
Igama lomkhiqizo
HWTS-UR018A-Herpes simplex virus uhlobo 1/2, (HSV1/2) ikhithi yokuthola i-nucleic acid (i-Fluorescence PCR)
I-Epidemiology
Izifo ezithathelwana ngocansi (STD) zisengenye yezinsongo ezinkulu ekuvikelekeni kwezempilo yomphakathi emhlabeni jikelele.Izifo ezinjalo zingaholela ekungazali, ukubeletha kwengane ngaphambi kwesikhathi, isimila kanye nezinkinga ezihlukahlukene ezinzima.Kunezinhlobo eziningi zamagciwane e-STD, okuhlanganisa amagciwane, amagciwane, i-chlamydia, i-mycoplasma ne-spirochetes, phakathi kwazo i-Neisseria gonorrhoeae, i-Mycoplasma genitalium, i-Chlamydia trachomatis, i-HSV1, i-HSV2, i-Mycoplasma hominis, ne-Ureaplasma urealyticum evamile.
I-Genital herpes yisifo esithathelwana ngocansi esivamile esibangelwa i-HSV2, esithathelwanayo kakhulu.Eminyakeni yamuva, izigameko ze-herpes zangasese ziye zanda kakhulu, futhi ngenxa yokwanda kokuziphatha kocansi okuyingozi, izinga lokutholwa kwe-HSV1 ku-herpes yobulili liye landa futhi kubikwa ukuthi liphezulu njenge-20% -30%.Ukutheleleka kokuqala ngegciwane le-herpes yangasese ngokuvamile kuthule ngaphandle kwezimpawu zomtholampilo ezisobala ngaphandle kwe-herpes yendawo ku-mucosa noma isikhumba seziguli ezimbalwa.Njengoba i-herpes yangasese ibonakala ngokuchithwa kwegciwane impilo yonke kanye nokuthambekela ekubuyeleni kabusha, kubalulekile ukuhlola amagciwane ngokushesha ngangokunokwenzeka futhi uvimbele ukudluliswa kwawo.
Isiteshi
FAM | HSV1 |
CY5 | HSV2 |
I-VIC(HEX) | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
Isitoreji | Uketshezi: ≤-18℃ ebumnyameni |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | ukukhishwa kwe-urethra, ukukhishwa komlomo wesibeletho |
Ct | ≤38 |
CV | ≤5.0% |
LoD | 50 Amakhophi/ukusabela |
Ukucaciswa | Akukho ukuhlangana kabusha namanye amagciwane e-STD afana ne-Treponema pallidum, i-Chlamydia trachomatis, i-Neisseria gonorrhoeae, i-Mycoplasma hominis, i-Mycoplasma genitalium, ne-Ureaplasma urealyticum. |
Izinsimbi Ezisebenzayo | Ingakwazi ukufanisa amathuluzi e-PCR e-fluorescent avamile emakethe. Applied Biosystems 7500 Fast Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems Uhlelo lwe-PCR lwe-LightCycler®480 Real-Time LineGene 9600 Plus Real-Time PCR Uhlelo Lokuthola I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit( HWTS-3001, HWTS-3004-32, HWTS-3004-48) kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor(HWTS-3006).
Inketho yesi-2.
Isisetshenziswa esisebenzayo sokukhipha esinconyiwe: Isizinda se-Nucleic Acid noma i-Purification Reagent(YDP302) yi-Tiangen Biotech(Beijing) Co.,Ltd.