Izinhlobo ezingama-28 ze-Human Papilloma Virus (16/18 Typing) enobungozi obukhulu beNucleic Acid
Igama lomkhiqizo
I-HWTS-CC006A-28 Izinhlobo Zegciwane Le-Human Papilloma Virus (16/18 Typing) I-Nucleic Acid Detection Kit (Fluorescence PCR)
I-Epidemiology
Umdlavuza womlomo wesibeletho ungesinye sezimila eziyingozi ezivame kakhulu emgudwini wokuzala wabesifazane.Ucwaningo luye lwabonisa ukuthi izifo ezihlala njalo ze-HPV kanye nezifo eziningi zingenye yezimbangela eziyinhloko zomdlavuza womlomo wesibeletho.Njengamanje, izindlela zokwelapha ezisebenzayo ezibonwayo azikatholakali umdlavuza womlomo wesibeletho obangelwa i-HPV, ngakho ukutholakala kusenesikhathi kanye nokuvinjelwa kokutheleleka komlomo wesibeletho okubangelwa yi-HPV kuwukhiye wokuvimbela umdlavuza womlomo wesibeletho.Kubaluleke kakhulu ukusungula isivivinyo sokuxilonga i-etiology elula, eqondile futhi esheshayo ukuze kuhlonzwe umtholampilo kanye nokwelashwa komdlavuza womlomo wesibeletho.
Isiteshi
Ukusabela Mix | Isiteshi | Uhlobo |
I-PCR-Mix1 | FAM | 18 |
I-VIC(HEX) | 16 | |
I-ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
CY5 | Ukulawula Kwangaphakathi | |
I-PCR-Mix2 | FAM | 6, 11, 54, 83 |
I-VIC(HEX) | 26, 44, 61, 81 | |
I-ROX | 40, 42, 43, 53, 73, 82 | |
CY5 | Ukulawula Kwangaphakathi |
Imingcele Yezobuchwepheshe
Isitoreji | Uketshezi: ≤-18℃ |
Impilo yeshelufu | Izinyanga ezingu-12 |
Uhlobo lwesifanekiso | iseli yomlomo wesibeletho exfoliated |
Ct | ≤28 |
CV | ≤5.0% |
LoD | 300Amakhophi/mL |
Izinsimbi Ezisebenzayo | I-Applied Biosystems 7500 Real-Time PCR Systems I-QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems I-LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems I-MA-6000 Real-Time Quantitative Thermal Cycler I-BioRad CFX96 Real-Time PCR System I-BioRad CFX Opus 96 Real-Time PCR System |
Ukugeleza komsebenzi
Inketho 1.
Ama-reagents okukhipha anconyiwe: I-Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48), kanye ne-Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).
Inketho yesi-2.
Ama-reagents okukhipha anconyiwe: I-Nucleic Acid Extraction noma Ikhithi Yokuhlanza(YDP315) yi-Tiangen Biotech (Beijing) Co., Ltd.